Lateral Flow Test

Raw Milk Mycotoxin Aflatoxin M1 Quantitative Rapid Test Kit

Aflatoxin M1 Quantitative Rapid Test Kit is a lateral flow assay that determines a quantitative level for the presence of aflatoxin M1 in raw cow’s milk. This test is designed for rapid field use or daily control in laboratories.

Sample Preparation

No sample preparation necessary.

Equipment Preparation

Turn on the Reader and warm up for 5minutes
Turnon the fully enclosed incubator and set the temperature to 30 ˚C
Beforetesting, the enclosed QR Code must been scanned just once for each kit lot to upload the information to the

Quality control preparation procedure

Add 2mL of deionized water into the negative control bottle; blend vigorously until the solid content dissolves completely.

Pipette 200μL of the prepared negative control into the positive control microwell, pipette up and down until the solid content dissolves completely.

Pipette200μL of the prepared positive control into the reaction microwell and follow the testing
Therecovery rate of the positive control is between 80-120%.
IMPORTANT:The reconstituted positive control should be used times.
Incubatethe sample for 3 minutes at 30˚C and then put one test strip into one
Letthe test strip develop color for 5 minutes at 30˚C.
Removethe absorbent pad and interpret test results within 1 minute.

Interpretation of the Results

Open the cassette, insert the quantitative test strip into the sample card slot, and ensure that the two bands on the surfaceof the white NC film are in the middle of the cassette
Insert cassette into Reader with cassette window facing up, andfollow the instrument's operating procedures, click TEST to get


Adhereto the instructions of test  Do not run more than 8 tests at one time.
Storetest kits at 2-8˚C when not in use, and do not use the kits beyond the expiration
Please don’t touch the membrane on the
Removemicrowell sealer slightly to prevent the powder from being released from the
Positivecontrol 1 bag
Negativecontrol 1 bottle
Pipette 1
Pipettetips 100
Microwellholder 1
QRcode 1

Materials Required but Not Provided:  100ng/kg

Order no.: SC0374

Detection Range (ng/kg=ppt)

Detection range: 0ng/kg-150ng/kg Limit of quantification: 20ng/kg

Kit Contents:

Name Specs Remark
Teststrips 12x8 strips 96 strips in total
Microwells 12x8wells 96 wells in total

Aflatoxin M1:

4 Strip reader


Scop of Application:  Raw cow’s milk

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