IAC for Zearalenone

1. Overview:

Zearalenone (ZEN), also known as F-2 toxin, is mainly produced by Fusarium strains. Its components are 2,4-dihydroxybenzoic acid lactones produced by Fusarium graminearum and Fusarium culmorum. ZEN mainly pollutes crops such as corn, wheat, and grains. It has a strong estrogenic effect and can cause hyperestrogen, as well as severe reproductive tract symptoms and infertility, and is also immunotoxin and genotoxic.

Product Properties: 2000ng;

Specification: 3mL,50T;

Smaples: Grain,feed,etc.

2. Principle:

The basis of the measurement is the antigen-antibody reaction. Antibodies are connected to the column and the toxins in the sample are extracted, filtered, and passed slowly through the zearalenone immunoaffinity column. The toxins bind to the antibodies in the column and the immunoaffinity column is then washed to remove other unrelated substances that have not been bound. Zearalenone is eluted with methanol, and then injected into the analytical instrument for detection.

3. Components of the kit:

Each kit contains Zearalenone immunoaffinity columns of various specifications and 1 instruction manual.

The immunoaffinity column can selectively adsorb zearalenone from the sample solution, thereby having a highly targeted purification effect on the sample. The sample solution that has been purified by passing through the column can be directly used for HPLC or LC-MS/MS analysis after concentrating with nitrogen gas and resuspended.

  • Allow the immunoaffinity column to return to room temperature (22 to 25°C) before use.
  • The immunoaffinity column should be stored at 2 to 8°C, do not freeze.
  • Dot use any expired immunoaffinity column.
  • The sample volume can be increased or decreased appropriately as required, and the volume of the extraction solution should be adjusted accordingly.
  • Column capacity: 2000 ng, when the content of the toxin in the sample divided by the dilution factor is higher than the column capacity, it is necessary to reduce the volume of the sample solution appropriately, and retest.
  • The pH of the loading solution of the immunoaffinity column should be within the range of 6 to 8. If it deviates from this range, the pH should be adjusted with dilute hydrochloric acid or dilute sodium hydroxide.
  • Maintaining consistency (such as polarity, pH, and concentration) between the test solvent loaded into any analytical instrument and the mobile phase can help eliminate any adverse solvent effects.
  • WARNING: Zearalenone is toxic and carcinogenic; protective equipment such as gloves and masks should always be used during handling.
  • Vessels and tools used to handle toxin solutions should be completely immersed in a sodium hypochlorite solution (5% v/v) overnight.
  • Ensure the LC-MS/MS is clean, and the tubing is primed appropriately for each run.
  • Follow appropriate instrument precautions if using HPLC.

Catalog No. Product Name Column Capacity (ng) Specification

HCM0125B/50B IAC for Aflatoxins Total B1,B2,G1,G2 300 3mL, 25T/50T

HCM0225B/50B IAC for Aflatoxins Total B1,B2,G1,G2,M1,M2 AFT B1,B2,G1,G2: 300 AFT M1: 100 AFT M2: 100 3mL, 25T/50T

HCM0325B/50B IAC for Aflatoxin B1 300 3mL, 25T/50T

HCM0525B/50B IAC for Zearalenone 2000 3mL, 25T/50T

HCM0625B/50B IAC for Deoxynivalenol 2000 3mL, 25T/50T

HCM3025B/50B IAC for Deoxynivalenols (DON/3-ADON/15-ADON) DON: 2000 3-ADON: 1000 15-ADON: 2000 3mL, 25T/50T

HCM0725B/50B IAC for Ochratoxin A 100 3mL, 25T/50T

HCM0825B/50B IAC for Fumonisins B1,B2,B3 5000 3mL, 25T/50T

HCM0925B/50B IAC for T-2 Toxin 2000 3mL, 25T/50T

HCM2025B/50B IAC for T-2,HT-2 Toxin 2000 3mL, 25T/50T

HCM2125B/50B IAC for Citrinin 500 3mL, 25T/50T

HCM2225B/50B IAC for Sterigmatocystin 600 3mL, 25T/50T

HCM3825/50 IAC 2-in-1 Aflatoxin B1/Deoxynivalenol IFU 3mL, 25T/50T

HCM3325B/50B IAC 2-in-1 Zearalenone/Fumonisins IFU 3mL, 25T/50T

HCM3525B/50B IAC 2-in-1 Zearalenone/Deoxynivalenol IFU 3mL, 25T/50T

HCM3725B/50B IAC 2-in-1 Aflatoxins (B1,B2,G1,G2)/Ochratoxin A IFU 3mL, 25T/50T

HCM4825B/50B IAC 2-in-1 Aflatoxin B1/Ochratoxin A IFU 3mL, 25T/50T

HCM5925B/50B IAC 2-in-1 Aflatoxin (B1,B2,G1,G2,M1,M2)/Ochratoxin A IFU 3mL, 25T/50T

HCM6025B/50B IAC 2-in-1 Zearalenone/Deoxynivalenols (DON,3-ADON, 15-ADON) IFU 3mL, 25T/50T

HCM6125B/50B IAC 2-in-1 Aflatoxins (B1,B2,G1,G2)/Zearalenone IFU 3mL, 25T/50T

HCM3125B/50B IAC 3-in-1 Aflatoxin B1/Zearalenone/Deoxynivalenol IFU 3mL, 25T/50T

HCM4125B/50B IAC 3-in-1 Aflatoxins (B1,B2,G1,G2)/Zearalenone/Deoxynivalenol IFU 3mL, 25T/50T

HCM6825B/50B IAC 3-in-1 Aflatoxins (B1,B2,G1,G2)/Zearalenone/Ochratoxin A IFU 3mL, 25T/50T

HCM4725B/50B IAC 3-in-1 Aflatoxin B1/Zearalenone/Ochratoxin A IFU 3mL, 25T/50T

HCM4925B/50B IAC 3-in-1 Zearalenone/Deoxynivalenol/Fumonisins IFU 3mL, 25T/50T

HCM6225B/50B IAC 3-in-1 Zearalenone/Deoxynivalenol/Ochratoxin A IFU 3mL, 25T/50T

HCM6525B/50B IAC 3-in-1 Aflatoxin B1/Zearalenone /T-2 Toxin IFU 3mL, 25T/50T

HCM3225B/50B IAC 4-in-1 Aflatoxin B1/Zearalenone/Deoxynivalenol/Ochratoxin A IFU 3mL, 25T/50T

HCM3625B/50B IAC 4-in-1 Aflatoxin B1/Zearalenone/Deoxynivalenols (DON,3- ADON,15-ADON)/Fumonisins IFU 3mL, 25T/50T

HCM6625B/50B IAC 4-in-1 Aflatoxins (B1,B2,G1,G2)/Ochratoxin A/Fumonisins/ Sterigmatocystin IFU 3mL, 25T/50T

HCM3110 IAC Mycotoxins 6-in-1 Combo IFU 6ml, 10T HCM4210 IAC Mycotoxins 7-in-1 Combo IFU 6mL,10T

Sample Type: Grain, oil, feed, herbs, etc.

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